Journal: Animal Bioscience
Article Title: Effect of cholesterol-loaded cyclodextrin treatment on boar sperm cryopreservation
doi: 10.5713/ab.24.0030
Figure Lengend Snippet: Effect of CLC-treatment on sperm protein consumption during freezing and thawing. The protein levels of CAPZB, PGAM2, and HSP90AA1 detected by western blotting (A). The quantification of CAPZB, PGAM2, and HSP90AA1 abundance detected by gray scanning (B–D). Values are presented as mean±standard deviation (n = 3). CLC, cholesterol-loaded cyclodextrin; CAPZB, capping actin protein of muscle Z-line subunit beta; PGAM2, phosphoglycerate mutase 2; HSP90AA1, heat shock protein 90 alpha family class A member 1. Control, no CLC treatment; low-CLC, 0.5 mg CLC/1.2×10 8 sperm; high-CLC, 1.0 mg CLC/1.2×10 8 sperm. ** p<0.01.
Article Snippet: , PGAM2 , Rabbit , 1:2,000 , Proteintech , 15550-1-AP.
Techniques: Western Blot, Standard Deviation, Control